Abstract
International Journal of Advance Research in Multidisciplinary, 2024;2(2):330-336
Assessment of genetic variability in Berberis lycium populations using ISSR and RAPD markers
Author : Saman Zahra Naqvi and Dr. Anubha Joshi
Abstract
Berberis lycium is a medicinally significant shrub widely distributed in the Himalayan region. Understanding its genetic diversity is crucial for conservation and sustainable utilization. In this study, we assessed the genetic variability among different populations of B. lycium using Inter Simple Sequence Repeat (ISSR) and Random Amplified Polymorphic DNA (RAPD) markers. A total of [X] ISSR primers and [Y] RAPD primers were screened, yielding a high level of polymorphism. The percentage of polymorphic bands (% PB) was recorded as [Z] % for ISSR markers and [W] % for RAPD markers, indicating substantial genetic variation. Cluster analysis using UPGMA grouped the populations into distinct clusters, reflecting genetic differentiation influenced by geographical and ecological factors. The high genetic diversity observed suggests that B. lycium maintains a broad genetic base, essential for adaptation and conservation strategies. This study provides a foundation for future genetic and breeding programs aimed at the sustainable management of B. lycium populations.
Keywords
Berberis lycium, genetic diversity, ISSR, RAPD, molecular markers, conservation