INTERNATIONAL JOURNAL OF ADVANCE RESEARCH
IN MULTIDISCIPLINARY

In this article, we detail how to modify Aedes aegypti at specified sites using the Cas9-CRISPR machinery. This approach utilizes RNA-DNA base-pairing to give targeted specificity, resulting in flexible and efficient genome-editing tools. We examine the efficacy of injection mix compositions, show that CRISPR-Cas9 may produce diverse mutations via distinct repair processes, and disclose persistent germ-line alterations in many genomic locations. Using Aedes aegypti as a model, this study delves further into the usage of CRISPR-Cas9, which might open the door to genetic alteration in non-model species.