Breeding and seed production of rohu, Labeo rohita, were carried out at Bhosi Village, Nirmal District, Telangana, to evaluate the practical use of GONOPRO-FH under farm conditions. The breeding operation took place during the monsoon season in an eco-hatchery using healthy, sexually mature brood fish more than two years old and weighing about 3 kg each. One female and two males formed a breeding unit in a 1:2 ratio. GONOPRO-FH was administered according to body weight at 0.4 mL kg⁻¹ to the female and 0.2–0.3 mL kg⁻¹ to the males. The brooders responded to hormonal induction and spawned successfully in the hatchery. The spawn were collected and transferred to a nursery pond prepared in advance. To promote natural food production, the nursery pond received 40 kg cow dung and 5 kg single super phosphate. Plankton developed after about six days and was checked with a plankton net before stocking. During breeding and nursery rearing, water temperature remained at 27–29 °C, pH at 7.0–8.0, dissolved oxygen at 7.5–8.0 mg L⁻¹, free carbon dioxide at 0.40–0.50 mg L⁻¹, and total alkalinity at 83–85 mg L⁻¹. Nursery survival was approximately 40%. Under these farm conditions, GONOPRO-FH supported successful rohu breeding when combined with mature brood stock, proper eco-hatchery operation, timely nursery preparation, plankton availability, and suitable water quality.