Understanding the ultrastructure of yeast organelles is fundamental for exploring cellular functions, biological pathways, and industrial applications. This paper focuses on the detailed architecture of key yeast organelles, including mitochondria, the nucleus, and vacuoles, utilizing advanced microscopy techniques such as transmission electron microscopy (TEM), scanning electron microscopy (SEM), and fluorescence microscopy. Comprehensive analyses revealed unique structural attributes, inter-organelle interactions, and dynamic adaptations of organelles to environmental and metabolic changes. The insights gained are crucial for applications in biotechnology, drug discovery, and molecular biology. The findings underscore the value of microscopy in unraveling the complexities of eukaryotic cell architecture.