This research paper analysed the link between the implementation of Treasury Single Account (TSA) and the total revenue collected by the federal government of Nigeria. The data used were collected from the CBN Statistical Bulletin and annual reports of various issues from the second quarter in 2011 up to the third quarter in 2019. The retrieved data were employed in running the analysis which involved descriptive statistics, mean difference test and the t-sampled test. The result of the study indicates that the implementation of TSA has a negative influence on the aggregated revenue collected by the federal government, however, it is statistically significant as the probability value is less than 0.05. It is therefore recommended that improvement be made to modify TSA to suite the Nigerian context, and that the federal government should diversify its revenue centers into agriculture and Information Communication Technology (ICT) in order to augment the total revenue and the government should establish more regulations and procedure to ensure accurate remita-keeping of receipts and revenue deposited into the TSA account.