The extant study pacts with the role of Persea Americana methanol and water extracts on microbial cultures. Persea Americana Methanol Extract (PAME) and Persea Americana water Extract (PAWE) were found to contain terpenoids, Glycosides, flavonoids, carbohydrates and steroids. But only PAME withholds polyphenol compounds, and PAWE contains only saponins, respectively in the extracts when it is subjected to preliminary phytoconstituent characterisation. Surprisingly, both PAME and PAWE confirm more than 10 peaks in analytical assays performed with GC-MS and HPLC instrumentation techniques. Which intern states that more phytochemical compounds are extracted in the extraction of PAME and PAWE. Importantly, PAME and PAWE contain several minerals like Aluminium, cadmium, zinc, barium, etc. Moreover, PAME and PAWE exhibit anti-microbial property by inhibiting the microbial culture growth, such as E. coli, S. aureus, pseudomonas, salmonella and Shigella species. Furthermore, PAME and PAWE were found to be non-toxic in nature as it unable to break RBC cells when we analyse an in-vitro study by using packed RBC.