The study titled “Status of Middle School Teachers in Serchhip District on Life Skill Education with regard to Gender and Subject Taught” aims to examine teachers’ understanding and implementation of life skill education. Life skill education is essential in fostering students’ emotional, social, and cognitive growth, equipping them to handle everyday challenges effectively. Teachers play a crucial role in promoting these skills within classrooms, making their knowledge and attitudes vital for holistic education. The study adopted a descriptive survey method using data collected from 65 middle school teachers across Serchhip district through the Kobo Collect application facilitated by the Magic Bus Foundation. Descriptive and inferential statistical tools such as mean, standard deviation, percentage, and t-test were used for analysis. The findings revealed that the overall status of teachers’ life skill education was moderate, indicating a balanced yet improvable understanding. A significant difference was found between male and female teachers, with females showing a higher level of awareness and application of life skill education. However, no significant difference was observed based on subject taught, suggesting a uniform approach across disciplines. The study underscores the need for continuous, gender-sensitive professional development programs to strengthen teachers’ capacity for effectively integrating life skill education in schools.